| 质粒名称: | pLKO-puro FLAG SREBP2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32018 |
| 存储实验室: | David Sabatini |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1-puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7100 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 将U6替换为CMV，并将标志添加到插入的N项中。5->3'：CMV->FLAG->sal I限制位点->1碱基（保留在框架中）->cDNA->Not I限制位点 |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SREBP2 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | FLAG（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SalI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLKO-puro FLAG SREBP2 was a gift from David Sabatini (Addgene plasmid # 32018 ; http://n2t.net/addgene:32018 ; RRID:Addgene_32018) |
| 参考文献部分: | mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLKO-puro FLAG SREBP2 10790 bp ds-DNA circular SYN 13-SEP-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLKO-puro FLAG SREBP2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10790)
AUTHORS Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas
E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM
TITLE mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP
Pathway.
JOURNAL Cell. 2011 Aug 5;146(3):408-20.
PUBMED 21816276
REFERENCE 2 (bases 1 to 10790)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 13, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10790
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 137..340
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 291..311
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 337..361
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 571..589
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 571..588
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 602..621
/label=pMT2-F
/note="Synthetic intron, forward primer"
CDS 680..703
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
misc_feature 4175..4292
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 4341..4851
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 4770..4788
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 4873..5472
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4873..4892)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5369..5389
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5600..5833
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5905..6026
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5942..5961)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5996..6015
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6066..6201
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6128..6147
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6221..6240)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6222..6240)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6250..6267)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6250..6266)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6259..6281)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6408..6863
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6495..6514)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6705..6726
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6889..6993
/gene="bla"
/label=AmpR promoter
CDS 6994..7854
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7212..7231)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8025..8613
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8514..8533
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8767..8784
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8901..8922
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8937..8967
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8975..8991
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8980..9002
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8999..9015
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8999..9015
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9034..9054
/label=T3
/note="T3 promoter, forward primer"
promoter 9036..9054
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 9082..9308
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 9309..9489
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 9536..9661
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 10158..10391
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 accccatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
61 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
121 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
181 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
241 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
301 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
361 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
421 cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga gtgacgtaag
481 taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg gctacaatta
541 atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa taacatccac
601 tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacctcgg ttctatcgat
661 tgaattcacc atggaaatgg actacaagga tgacgatgac aagggtggtg gtggtggtgc
721 gtcgacgatg gatgagagca gcgagctggg cgttctggag accatggaga ccctcacgga
781 gctgggcgat gagctgactc tcggggacat cgacgagatg ctacagtttg tcagcaatca
841 agtgggagag ttccctgatt tgttttcaga gcaactttgt agctccttcc ctggaggtgg
901 cagcaatggt ggcagtggca acaacagcag tggcagaggc aacaatggtg gagccacaga
961 ccctgcagta cagcggtcat tcagccaggt cccattatct accttctctc cctcagctgc
1021 ctcccctcag gctccggccc tgcaggtcaa agtctctcct accccaccaa gggcaacccc
1081 tgttctccag ccgcgacccc agccccagcc ccaacctcca gctcagctgc agcagcagac
1141 ggtgatgatc accccgacgt tcagcaccgc tccgcagacg aggatcatcc agcagccttt
1201 gatataccag aacgcagcga ccagctttca agtcctgcag cctcaagtgc aaagcctcgt
1261 gacgtccccg caggtgcagc ccgtcaccat ccagcagcag gtgcagacgg tacaggccca
1321 gcgggtgttg acgcagacag ccaatggcac actgcagacc cttgcccccg ccacggtaca
1381 gacggtcgct gcgccacagg tgcagcaggt cccggtcctg gtgcaacctc agatcattaa
1441 gacagattcc cttgtgttga ccacgctgaa gacagatggc agccctgtca tggctgcagt
1501 ccagaacccg gccctcacgg ccctcactgc ccccatccag acagccgccc ttcaagtgcc
1561 aaccctggtg ggcagcaacg ggaccattct gaccacaatg cctgtgatga tggggcaaga
1621 gaaagttcct atcaagcaag tgcctggcgg cgtgaagcag ctggatcctc ccaaagaagg
1681 agagaggcgg acaacacaca atatcattga aaagcgctac cggtcctcca tcaacgacaa
1741 aatcatagag ttgaaggact tagtcatggg gacagatgcc aagatgcaca agtctggcgt
1801 tctgaggaag gccattgatt acatcaaata tctgcagcag gtcaatcaca agctgcgcca
1861 ggagaacatg gtgctgaagc tggccaatca gaaaaacaag ctcctgaagg gcatcgacct
1921 gggcagtctg gtggacagtg atgtggactt gaaaattgat gactttaacc agaatgtcct
1981 tctgatgtct ccgccggcct ccgactccgg gtcccaggcc ggcttctctc cctattccat
2041 tgactctgag ccgggcagcc ctctgctgga tgacgcaaag gtcaaggatg aaccggactc
2101 tcctcctgtg gcactgggca tggtggaccg ctctcgaatc ctcttatgtg tcctcacctt
2161 cctgggcctc tcctttaacc ccttgacttc cttgctgcag tggggagggg cccacaacac
2221 tgaccagcac ccatactcag gctcgggccg cagtgtactg tcactggagt caggtgctgg
2281 gggctggttt gactggatgg ttccaactct cctcctgtgg ctggtaaatg gtgtgattgt
2341 cttgagcgtc tttgtgaagc tgttggtcca cggggagccg gtgattcgcc cacactcacg
2401 cccctcagtc accttctgga gacaccggaa gcaggcagac ctagacctcg ccaaaggtga
2461 tttcgcagct gctgctgcca acctacaaac ctgcttatcc gtgctgggcc gggcgctgcc
2521 cacctcccgc ctggacttgg cctgcagcct ctcctggaat gtgatccgct acagcctgca
2581 gaagctgcgc ctggtacgct ggttactcaa gaaggtcttc cagcgctggc gggctactcc
2641 cgccactgca gctggctttg aggatgaggc taagagcagc gcaagggatg cggcactggc
2701 ctaccacagg ctgcaccagc tgcacatcac agggaagctt ccggcgggat ctgcctgttc
2761 agatgtgcac atggctctgt gtgctgtgaa cctggccgag tgtgcggagg agaaaatcct
2821 accaagcaca ctgattgaga tccacctgac agcggccatg gggctcaaga cccgctgtgg
2881 aggcaagctg ggcttcttgg ctagctactt cttaaatcgt gcccagagtc tgtgtggccc
2941 agagcatagc actgtccccg actccctgcg ctggctatgc catcccttgg gccagaagtt
3001 tttcatggag cgaagctggt cgatcaagtc agcagccaag gagagcctgt actgcgccca
3061 gaggagccca gctgacccca ttgcccaggt acaccaggcg ttctgcaaaa acctgctaga
3121 aagagcggtg gagtccttgg tgaaacctca ggccaagaag aaggcaggcg accaggaaga
3181 agagagctgt gaattttcca gtgctctaga gtatttgaaa ttacttcatt cttttgtgga
3241 ctctgtggga tttgtgacct caccattctc cagcagttcc gtgctcaggt cggcccttgg
3301 ccccgatgtc atctgccggt ggtggacgtc cgcagtcacc atggccatca gctggctcca
3361 gggagacgat gcagctgtgc gctctcgttt tactgaagta gagcgggtcc ccaaggccct
3421 ggaagtgacc gagagtccct tggtgaaggc cgtcttctat acctgtagag ccatgcatgc
3481 ctccctctct gggaaagctg acgggcagca gaactccttc tgtcactgtg agagggccag
3541 tggtcacctg tggagcagtc tcaacgtcag cggcactact tctgatcctt cacttaacca
3601 tgtgatccag ctgttcacct gtgacctgct actgtcgctg aggacagcac tctggcaaaa
3661 gcaggccagt gccagccagc tcctgggtga gacctaccat gcatccggca ctgagctggc
3721 cggcttccag cgggacctgg gcagccttcg aaggctggcc catagcttcc gcccagccta
3781 ccgcaaggtg ttcctgcatg aagccactgt gcgcctgatg gcaggagcca gccctacccg
3841 tacacaccag ctgctggagc atagcctacg gcggcgcccc actcagaaca ccaagcatgg
3901 agaggtagac acctggcctg gccaacgaga gcgagctact gccatcctgc tggcctgccg
3961 ccatcttccc ctctctttcc tgtcttcccc gggccagcgg gcagtgctgc tggctgaagc
4021 tgcccgcacc ctggagaagg tgggtgaccg ccgttcctgc agtgactgcc agcagatgat
4081 tgtcaagctg ggcggtggca ctgccatcgc tgcctcctga gcggccgcag tgagaattct
4141 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
4201 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
4261 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
4321 cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
4381 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
4441 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
4501 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
4561 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
4621 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
4681 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
4741 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
4801 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca
4861 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
4921 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
4981 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
5041 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
5101 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
5161 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
5221 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
5281 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
5341 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
5401 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
5461 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
5521 catgcatcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
5581 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
5641 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
5701 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
5761 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
5821 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
5881 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
5941 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
6001 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
6061 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
6121 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
6181 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
6241 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
6301 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
6361 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
6421 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
6481 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
6541 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
6601 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
6661 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
6721 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
6781 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
6841 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
6901 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
6961 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
7021 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
7081 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
7141 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
7201 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
7261 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
7321 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
7381 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
7441 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
7501 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
7561 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
7621 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
7681 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
7741 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
7801 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
7861 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
7921 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
7981 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
8041 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
8101 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
8161 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
8221 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
8281 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
8341 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
8401 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
8461 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
8521 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
8581 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
8641 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
8701 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
8761 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
8821 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
8881 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
8941 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
9001 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
9061 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
9121 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
9181 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
9241 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
9301 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
9361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
9421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
9481 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
9541 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
9601 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
9661 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
9721 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
9781 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
9841 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
9901 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
9961 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
10021 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
10081 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
10141 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
10201 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
10261 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
10321 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
10381 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
10441 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
10501 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
10561 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
10621 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
10681 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
10741 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
//
